MELK 抗体 (C-Term)
Quick Overview for MELK 抗体 (C-Term) (ABIN374073)
抗原
See all MELK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- KRLVEDILSS CKV
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特异性
- This antibody detects MELK at C-term.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Canine (Dog), Cow.
Species reactivity (tested):Human. -
纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Syntehtic Peptide from the C Terminus of the protein sequence according to NP_055606.1. Genename: MELK
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anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 637-651) antibody
MELK 适用: 人 ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) antibodyMELK 适用: 人 ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 637-651) antibodyMELK 适用: 人 ELISA, IHC, FACS, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 434-468), (C-Term) antibodyMELK 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB51204 unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) antibodyMELK 适用: 人 IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (Center) antibodyMELK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 101-250) antibodyMELK 适用: 人 ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) (AA 412-651) antibodyMELK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) antibodyMELK 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Maternal Embryonic Leucine Zipper Kinase (MELK) antibodyMELK 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3´μg/mL. Experiments gave bands at approx 75 kDa and 150 kDa inhuman testis lysates and in cell lines HeLa and Jurkat after 0.1 μg/mL antibody staining. The 150 kDa band is explained by glycosylation of this member of the AMPK family. Thisprotein has a calculated MW of 74.6 kDa according to NP_055606.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 containng 0.02 % Sodium Azide and 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MELK (Maternal Embryonic Leucine Zipper Kinase (MELK))
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别名
- MELK
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背景
- MELK, maternal embryonic leucine zipper kinase, belongs to the protein kinase superfamily. It contains 1 protein kinase domain and is expressed in placenta, kidney, thymus, testis, ovary and intestine. MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. It may also be involved in the inhibition of spliceosome assembly during mitosis.Synonyms: KIAA0175, Maternal embryonic leucine zipper kinase, hMELK
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基因ID
- 9833
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NCBI登录号
- NP_055606
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UniProt
- Q14680
抗原
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